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|Title:||Effect of combined treatment of hydrolysis and polymerization with transglutaminase on b-lactoglobulin antigenicity|
|Author:||Sabadin, Isabele Serimarco|
Villas-Boas, Mariana Battaglin
Zollner, Ricardo de Lima
Netto, Flavia Maria
|Abstract:||The effect of combined treatments of hydrolysis with different proteases, and subsequent polymerization with transglutaminase on the antigenic activity of β-Lg was studied. For the hydrolysis of β-Lg using Alcalase, Neutrase or bromelain, the reaction conditions were 3 % β-Lg and enzyme:substrate 25 U g−1 of protein, as was defined using factorial study. Under these conditions, the degree of hydrolysis (DH) of the hydrolysates was 12.6 % when obtained with Alcalase and approximately 4 % with Neutrase or bromelain. Post-hydrolysis polymerization did not result in an increase in molecular mass of the protein, but these samples presented a lower DH, determined by trinitrobenzenosulfonic acid (TNBS) method, suggesting that polymerization had occurred. Hydrolysis with the three enzymes reduced the β-Lg antigenicity, as evaluated by ELISA and immunoblotting analyses. The IgE-binding responses were practically null (<9 μg mL−1), 22.82 and 55.73 μg mL−1 towards the hydrolysates obtained with Alcalase, bromelain, and Neutrase, respectively. The post-hydrolysis polymerization increased or had no significant effect (P ≥ 0.05) on the antigenic response of the hydrolysates|
|Appears in Collections:||FCM - Artigos e Outros Documentos|
FEA - Artigos e Outros Documentos
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