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Type: Artigo de periódico
Title: Strand Analysis, a free online program for the computational identification of the best RNA interference (RNAi) targets based on Gibbs free energy
Author: Pereira, Tiago Campos
Bittencourt, Vinícius D'Ávila Pascoal
Secolin, Rodrigo
Rocha, Cristiane de Souza
Maia, Ivan de Godoy
Lopes-Cendes, Iscia
Abstract: The RNA interference (RNAi) technique is a recent technology that uses double-stranded RNA molecules to promote potent and specific gene silencing. The application of this technique to molecular biology has increased considerably, from gene function identification to disease treatment. However, not all small interfering RNAs (siRNAs) are equally efficient, making target selection an essential procedure. Here we present Strand Analysis (SA), a free online software tool able to identify and classify the best RNAi targets based on Gibbs free energy (deltaG). Furthermore, particular features of the software, such as the free energy landscape and deltaG gradient, may be used to shed light on RNA-induced silencing complex (RISC) activity and RNAi mechanisms, which makes the SA software a distinct and innovative tool.
Subject: RNAi
siRNA design
Editor: Sociedade Brasileira de Genética
Citation: Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 30, n. 4, p. 1206-1208, 2007.
Rights: aberto
Identifier DOI: 10.1590/S1415-47572007000600030
Date Issue: 1-Jan-2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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