Please use this identifier to cite or link to this item:
|Title:||Nanotoxicity and dermal application of nanostructured lipid carrier loaded with hesperidin from orange residue|
Costa, Amanda F.
Bernardes, Juliana S.
Tasic, Ljubica T
|Abstract:||Flavonoids are a class of polyphenolic compounds that can be extracted from citrus fruits. Because of their features, they have been considered as new bullets in cancer therapy. Flavonoids present anti-oxidant, anti-inflammatory and anti-cancer properties and some studies are pointing that their higher intake involves lower risks of cancer. The nanostructured lipid carriers (NLC) are drug delivery systems composed from a mixture of solid and liquid lipids that increase physical stability and promote the sustained release of the drug. So, the main aim of this research was to combine the potential of the flavonoid as a natural anti-oxidant compound with the nanostructured lipid carriers to form an efficient system for flavonoid delivery into the cells. These NLC were prepared through high pressure homogenization technique and were evaluated in function of several standard properties. The samples exhibited good stability through time and temperature, with sizes of around 215 nm and zeta potentials of -35 mV during a period of 105 days. The encapsulation and the loading efficiency were respectively 96% and 2.25% up to 105 days of storage. In vitro release assays showed a burst process for NLC-L pointing to their shell structure with flavonoid situated in the contour of the particles. The cell viability tests showed that flavonoid, either free or loaded into NLC, did not present in vitro toxicity to the melanoma cells tested: A375, CHL01 and SKMEL147 through 3-(4,5-dimethyiltiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT), neutral red and crystal violet assays. Hesperidin at 45 μmol L−1 was able to reduce significantly the radical forming up to 92.58 ± 0.82% in DPPH assay. On the other hand, the produced NLC were applied in a skin lotion formulation, which showed a good stability and opened up a new application for the produced nanoparticles as in anti-aging and moisturizing cosmetics. Finally, the in vitro cytotoxicity tests were carried out using several human culture cells. The flavonoid loaded and empty nanocarriers have been used to treat the cells. All data suggested that, at the level studied, non-significant toxicities on cells were observed|
|Appears in Collections:||IQ - Artigos e Outros Documentos|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.