Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/325923
Type: Artigo
Title: Chronic Treatment With Resveratrol Improves Overactive Bladder In Obese Mice Via Antioxidant Activity
Chronic treatment with resveratrol improves overactive bladder in obese mice via antioxidant activity
Author: Alexandre, Eduardo C.
Calmasini, Fabiano B.
Oliveira, Mariana G. de
Silva, Fabio H.
Silva, Carmem P. V. da
Andre, Diana M.
Leonardo, Flavia C.
Delbin, Maria A.
Antunes, Edson
Abstract: The objective of the present work was to evaluate whether oral intake with resveratrol ameliorates overactive bladder in high-fat fed mice. Male C57BL6 mice fed with standard chow or high-fat diet to induce obesity received a two-week therapy with resveratrol (100 mg/kg, given as a daily gavage). Weight and metabolic profile, together with cystometry and in vitro bladder contractions were evaluated. Measurements of gp91phox and SOD1 mRNA expressions and reactive-oxygen species (ROS) in bladder tissues, and serum TBARS were performed. Obese mice exhibited increases in body weight and epididymal fat mass, which were significantly reduced by oral treatment with resveratrol. Cystometric study in obese mice showed increases in non-voiding contractions, post-voiding pressure and voiding frequency that were reversed by resveratrol treatment. Likewise, the in vitro bladder overactivity in response to electrical-field stimulation (80 V, 1-32 Hz) or carbachol (1 nM to 10 mM) were normalized by resveratrol. The gp91phox and SOD1 mRNA expressions in bladder tissues were markedly higher in obese mice compared with lean group. In addition, ROS levels in bladder tissues and serum lipid per oxidation (TBARS assay) were markedly higher in obese compared with lean mice, all of which were reduced by resveratrol treatment. In lean group, resveratrol had no effect in any parameter evaluated. Our results show that two-week therapy of obese mice with resveratrol reduces the systemic and bladder oxidative stress, and greatly ameliorated the cystometry alterations and in vitro bladder overactivity. Resveratrol treatment could be an option to prevent obesity-associated overactive bladder. (C) 2016 Elsevier B.V. All rights reserved.
The objective of the present work was to evaluate whether oral intake with resveratrol ameliorates overactive bladder in high-fat fed mice. Male C57BL6 mice fed with standard chow or high-fat diet to induce obesity received a two-week therapy with resveratrol (100 mg/kg, given as a daily gavage). Weight and metabolic profile, together with cystometry and in vitro bladder contractions were evaluated. Measurements of gp91phox and SOD1 mRNA expressions and reactive-oxygen species (ROS) in bladder tissues, and serum TBARS were performed. Obese mice exhibited increases in body weight and epididymal fat mass, which were significantly reduced by oral treatment with resveratrol. Cystometric study in obese mice showed increases in non-voiding contractions, post-voiding pressure and voiding frequency that were reversed by resveratrol treatment. Likewise, the in vitro bladder overactivity in response to electrical-field stimulation (80 V, 1-32 Hz) or carbachol (1 nM to 10 mM) were normalized by resveratrol. The gp91phox and SOD1 mRNA expressions in bladder tissues were markedly higher in obese mice compared with lean group. In addition, ROS levels in bladder tissues and serum lipid per oxidation (TBARS assay) were markedly higher in obese compared with lean mice, all of which were reduced by resveratrol treatment. In lean group, resveratrol had no effect in any parameter evaluated. Our results show that two-week therapy of obese mice with resveratrol reduces the systemic and bladder oxidative stress, and greatly ameliorated the cystometry alterations and in vitro bladder overactivity. Resveratrol treatment could be an option to prevent obesity-associated overactive bladder
Subject: Cystometry
Gp91phox
Nadph Oxidase
Obesity
Reactive-oxygen Species
Superoxide Dismutase
NADPH oxidase
Obesidade
Superóxido dismutase
Country: Países Baixos
Editor: Elsevier
Citation: Euroepan Journal Of Pharmacology. Elsevier Science Bv, v. 788, p. 29 - 36, 2016.
Rights: fechado
Identifier DOI: 10.1016/j.ejphar.2016.06.017
Address: https://www.sciencedirect.com/science/article/pii/S0014299916303776
Date Issue: 2016
Appears in Collections:IB - Artigos e Outros Documentos
FCM - Artigos e Outros Documentos
CPQBA - Artigos e Outros Documentos

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