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Type: Artigo de Periódico
Title: Inhibitory Effects Of Compounds Isolated From The Dried Branches And Leaves Of Murta (myrceugenia Euosma) On Lipid Accumulation In 3t3-l1 Cells
Author: Oikawa
N; Nobushi
Y; Wada
T; Sonoda
K; Okazaki
Y; Tsutsumi
S; Park
YK; Kurokawa
M; Shimba
S; Yasukawa
Abstract: As obesity is a global health concern the demand for anti-obesity drugs is high. In this study, we investigated the anti-obesity effect of the dried branches and leaves of murta (Myrceugenia euosma Legrand, Myrtaceae). A methanol extract of the dried branches and leaves of murta inhibited adipogenesis in 3T3-L1 cells. Three known flavanones-cryptostrobin (1), pinocembrin (4), and 5,7-dihydroxy-6,8-dimethylflavanone (6), and three chalcones-2',6'-dihydroxy-3'-methyl-4'-methoxychalcone (2), pinostrobin chalcone (3), and 2',6'-dihydroxy-4'-methoxy-3',5'-dimethylchalcone (5) were isolated from the active fraction. Structures of these compounds were identified using various spectral data. Each of these compounds also inhibited adipogenesis in 3T3-L1 cells. In particular, compound 3 was a more potent inhibitor of triglyceride accumulation than the positive control berberine. Gene expression studies revealed that treatment of 3T3-L1 cells with 3 lowers the expression levels of CCAAT/enhancer-binding protein alpha and peroxisome proliferator activator gamma 2 during adipogenesis without affecting cell viability. Treatment of 3T3-L1 cells with 3 reduced the expression levels of mRNAs encoding sterol regulatory element-binding protein 1c and several lipogenic enzymes, including fatty acid synthase and stearoyl CoA desaturase-1. These results indicate that the methanol extract and compounds isolated from the dried branches and leaves of murta exert their anti-obesity effects through the inhibition of adipogenesis.
Subject: Murta
3t3-l1 Cells
Lipid Accumulation
Metabolic Syndrome
Citation: Journal Of Natural Medicines. SPRINGER JAPAN KK, n. 70, n. 3, p. 502 - 509.
Rights: fechado
Identifier DOI: 10.1007/s11418-016-0968-2
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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