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Type: Artigo de Periódico
Title: Methylmercury Degradation By Pseudomonas Putida V1
Author: Cabral
L; Yu
RQ; Crane
S; Giovanella
P; Barkay
T; Camargo
Abstract: Environmental contamination of mercury (Hg) has caused public health concerns with focuses on the neurotoxic substance methylmercury, due to its bioaccumulation and biomagnification in food chains. The goals of the present study were to examine: (i) the transformation of methylmercury, thimerosal, phenylmercuric acetate and mercuric chloride by cultures of Pseudomonas putida VI, (ii) the presence of the genes merA and merB in P. putida V1, and (iii) the degradation pathways of methylmercury by P. putida VI. Strain V1 cultures readily degraded methylmercury, thimerosal, phenylmercury acetate, and reduced mercuric chloride into gaseous Hg(0). However, the Hg transformation in LB broth by P. putida V1 was influenced by the type of Hg compounds. The merA gene was detected in P. putida V1, on the other hand, the merB gene was not detected. The sequencing of this gene, showed high similarity (100%) to the mercuric reductase gene of other Pseudomonas spp. Furthermore, tests using radioactive C-14-methylmercury indicated an uncommon release of (CO2)-C-14 concomitant with the production of Hg. The results of the present work suggest that P. putida V1 has the potential to remove methylmercury from contaminated sites. More studies are warranted to determine the mechanism of removal of methylmercury by P. putida V1. (C) 2016 Elsevier Inc. All rights reserved.
Subject: Methylmercury
Mer Operon
P. Putida
Contaminated Sites
Citation: Ecotoxicology And Environmental Safety. ACADEMIC PRESS INC ELSEVIER SCIENCE, n. 130, p. 37 - 42.
Rights: fechado
Identifier DOI: 10.1016/j.ecoenv.2016.03.036
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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