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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titlePlatelet antibody detection by flow cytometry: an effective method to evaluate and give transfusional support in platelet refractorinesspt_BR
dc.contributor.authorBub, Carolina Bonetpt_BR
dc.contributor.authorMartinelli, Beatriz Moraespt_BR
dc.contributor.authorAvelino, Thayna Mendoncapt_BR
dc.contributor.authorGoncalez, Ana Claudiapt_BR
dc.contributor.authorBarjas-Castro, Maria de Lourdespt_BR
dc.contributor.authorCastro, Vagnerpt_BR
unicamp.authorBub, Carolina Bonet Universidade Estatual de Campinas Estadual de Campinaspt_BR
unicamp.authorMartinelli, Beatriz Moraes Universidade Estatual de Campinas Estadual de Campinaspt_BR
unicamp.authorAvelino, Thayna Mendonca Universidade Estatual de Campinas Estadual de Campinaspt_BR
unicamp.authorGoncalez, Ana Claudia Universidade Estatual de Campinas Estadual de Campinaspt_BR
unicamp.authorBarjas-Castro, Maria de Lourdes Universidade Estatual de Campinas Estadual de Campinaspt_BR
unicamp.authorCastro, Vagner Universidade Estatual de Campinas Estadual de Campinaspt_BR
dc.subjectBlood plateletspt_BR
dc.subjectAntigens, human leukocytept_BR
dc.subjectFlow cytometrypt_BR
dc.subjectHistocompatibilitypt_BR
dc.subjectAntigens, human plateletpt_BR
dc.description.abstractBACKGROUND: Immune platelet refractoriness is mainly caused by human leukocyte antigen antibodies (80-90% of cases) and, to a lesser extent, by human platelet antigen antibodies. Refractoriness can be diagnosed by laboratory tests and patients should receive compatible platelet transfusions. A fast, effective and low cost antibody-screening method which detects platelet human leukocyte/platelet antigen antibodies is essential in the management of immune platelet refractoriness.OBJECTIVE: The aim of this study was to evaluate the efficiency of the flow cytometry platelet immunofluorescence test to screen for immune platelet refractoriness.METHODS: A group of prospective hematologic patients with clinically suspected platelet refractoriness treated in a referral center in Campinas, SP during July 2006 and July 2011 was enrolled in this study. Platelet antibodies were screened using the flow cytometry platelet immunofluorescence test. Anti-human leukocyte antigen antibodies were detected by commercially available methods. The sensitivity, specificity and predictive values of the immunofluorescence test were determined taking into account that the majority of antiplatelet antibodies presented human leukocyte antigen specificity.RESULTS: Seventy-six samples from 32 female and 38 male patients with a median age of 43.5 years (range: 5-84 years) were analyzed. The sensitivity of the test was 86.11% and specificity 75.00% with a positive predictive value of 75.61% and a negative predictive value of 85.71%. The accuracy of the method was 80.26%.CONCLUSION: This study shows that the flow cytometry platelet immunofluorescence test has a high correlation with the anti-human leukocyte antigen antibodies. Despite a few limitations, the method seems to be efficient, fast and feasible as the initial screening for platelet antibody detection and a useful tool to crossmatch platelets for the transfusional support of patients with immune platelet refractoriness.en
dc.relation.ispartofRevista Brasileira de Hematologia e Hemoterapiapt_BR
dc.publisherAssociação Brasileira de Hematologia e Hemoterapia e daSociedade Brasileira de Transplante de Medula Ósseapt_BR
dc.date.issued2013-01-01pt_BR
dc.identifier.citationRevista Brasileira de Hematologia e Hemoterapia. Associação Brasileira de Hematologia e Hemoterapia e daSociedade Brasileira de Transplante de Medula Óssea, v. 35, n. 4, p. 252-255, 2013.pt_BR
dc.language.isoenpt_BR
dc.description.firstpage252pt_BR
dc.description.lastpage255pt_BR
dc.rightsabertopt_BR
dc.sourceSciELOpt_BR
dc.identifier.issn1516-8484pt_BR
dc.identifier.idScieloS1516-84842013000400252pt_BR
dc.identifier.doi10.5581/1516-8484.20130062pt_BR
dc.identifier.urlhttp://dx.doi.org/10.5581/1516-8484.20130062pt_BR
dc.identifier.urlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842013000400252pt_BR
dc.date.available2014-07-17T17:54:28Z
dc.date.available2015-11-26T11:57:00Z-
dc.date.accessioned2014-07-17T17:54:28Z
dc.date.accessioned2015-11-26T11:57:00Z-
dc.description.provenanceMade available in DSpace on 2014-07-17T17:54:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-01-01en
dc.description.provenanceMade available in DSpace on 2015-11-26T11:57:00Z (GMT). No. of bitstreams: 2 S1516-84842013000400252.pdf: 408097 bytes, checksum: 046e4540eb2eaa4f1b5c92bde196e207 (MD5) S1516-84842013000400252.pdf.txt: 18645 bytes, checksum: dbd54aebc0c01d8ebed94e7d24bf5129 (MD5) Previous issue date: 2013-01-01en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/27313
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/27313-
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