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Type: Artigo de periódico
Title: Evaluation Of Enzyme Immobilization Methods For Paper-based Devices-a Glucose Oxidase Study
Author: Nery
Emilia Witkowska; Kubota
Lauro T.
Abstract: Paper-based sensors gained almost explosive attention during the last few years. A large number of systems, often destined to resource limited settings is based on enzymatic reactions. Choice of an adequate immobilization method could significantly prolong the shelf-life of such sensors, especially in applications, where exposure to high temperatures during storage and transport is more than a threat. We are seeking to compare a variety of immobilization methods based on different phenomena (adsorption, entrapment in gel, microencapsulation, covalent linkage), with total of 33 methods tested. Glucose oxidase was used as a model enzyme. Enzymatic activity of immobilized samples was accompanied for a period of 24 weeks considering two sets of samples, one stored in 4 degrees C and other in ambient temperature. (C) 2015 Elsevier B.V. All rights reserved.
Subject: Layer-by-layer
Citation: Evaluation Of Enzyme Immobilization Methods For Paper-based Devices-a Glucose Oxidase Study. Elsevier Science Bv, v. 117, p. 551-559 Jan-2016.
Rights: embargo
Identifier DOI: 10.1016/j.jpba.2015.08.041
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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