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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleTrypanosoma cruzi: parasite persistence in tissues in chronic chagasic Brazilian patientspt_BR
dc.contributor.authorMarcon, Gláucia E Barbosapt_BR
dc.contributor.authorAlbuquerque, Dulcinéia Martins dept_BR
dc.contributor.authorBatista, Angelica Martinspt_BR
dc.contributor.authorAndrade, Paula Durantept_BR
dc.contributor.authorAlmeida, Eros Apt_BR
dc.contributor.authorGuariento, Maria Elenapt_BR
dc.contributor.authorTeixeira, Maria ABpt_BR
dc.contributor.authorCosta, Sandra C Botelhopt_BR
unicamp.authorMarcon, Gláucia E Barbosa Universidade Estatual de Campinas Estadual de Campinas Faculdade de Ciências Médicas Departamento de Clínica Médicapt_BR
unicamp.authorAlbuquerque, Dulcinéia Martins de Universidade Estatual de Campinas Estadual de Campinas Faculdade de Ciências Médicas Departamento de Clínica Médicapt_BR
unicamp.authorBatista, Angelica Martins Universidade Estatual de Campinas Estadual de Campinas Faculdade de Ciências Médicas Departamento de Clínica Médicapt_BR
unicamp.authorAndrade, Paula Durante Universidade Estatual de Campinas Estadual de Campinas Faculdade de Ciências Médicas Departamento de Clínica Médicapt_BR
unicamp.authorAlmeida, Eros A Universidade Estatual de Campinas Estadual de Campinas Faculdade de Ciências Médicas Departamento de Clínica Médicapt_BR
unicamp.authorGuariento, Maria Elena Universidade Estatual de Campinas Estadual de Campinas Faculdade de Ciências Médicas Departamento de Clínica Médicapt_BR
unicamp.authorTeixeira, Maria ABpt_BR
unicamp.authorCosta, Sandra C Botelho Universidade Estatual de Campinas Estadual de Campinas Faculdade de Ciências Médicas Departamento de Clínica Médicapt_BR
dc.subjectTrypanosoma cruzipt_BR
dc.subjectreal-time PCRpt_BR
dc.subjectnested PCRpt_BR
dc.subjectChagas diseasept_BR
dc.description.abstractChagas disease in the chronic phase may develop into cardiac and/or digestive forms. The pathogenesis of the disease is not yet clear and studies have been carried out to elucidate the role of parasite persistence in affected organs. The aim of this study was to detect and quantify Trypanosoma cruzi in paraffin-embedded tissue samples from chronic patients using NPCR (nested polymerase chain reaction) and QPCR (quantitative polymerase chain reaction) methods. These results were correlated to anatomopathological alterations in the heart and gastrointestinal tract (GIT). Of the 23 patients studied, 18 presented the cardiac form and five presented the cardiodigestive form of Chagas disease. DNA samples were randomly isolated from formalin-fixed paraffin-embedded sections of heart and GIT tissue of 23 necropsies and were analyzed through NPCR amplification. T. cruzi DNA was detected by NPCR in 48/56 (85.7%) heart and 35/42 (83.3%) GIT samples from patients with the cardiac form. For patients with the cardiodigestive form, NPCR was positive in 12/14 (85.7%) heart and in 14/14 (100%) GIT samples. QPCR, with an efficiency of 97.6%, was performed in 13 samples (11 from cardiac and 2 from cardiodigestive form) identified previously as positive by NPCR. The number of T. cruzi copies was compared to heart weight and no statistical significance was observed. Additionally, we compared the number of copies in different tissues (both heart and GIT) in six samples from the cardiac form and two samples from the cardiodigestive form. The parasite load observed was proportionally higher in heart tissues from patients with the cardiac form. These results show that the presence of the parasite in tissues is essential to Chagas disease pathogenesis.en
dc.relation.ispartofMemórias do Instituto Oswaldo Cruzpt_BR
dc.publisherInstituto Oswaldo Cruz, Ministério da Saúdept_BR
dc.date.issued2011-02-01pt_BR
dc.identifier.citationMemórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 106, n. 1, p. 85-91, 2011.pt_BR
dc.language.isoenpt_BR
dc.description.firstpage85pt_BR
dc.description.lastpage91pt_BR
dc.rightsabertopt_BR
dc.sourceSciELOpt_BR
dc.identifier.issn0074-0276pt_BR
dc.identifier.idScieloS0074-02762011000100014pt_BR
dc.identifier.doi10.1590/S0074-02762011000100014pt_BR
dc.identifier.urlhttp://dx.doi.org/10.1590/S0074-02762011000100014pt_BR
dc.identifier.urlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000100014pt_BR
dc.date.available2014-07-16T20:38:08Z
dc.date.available2015-11-26T11:45:28Z-
dc.date.accessioned2014-07-16T20:38:08Z
dc.date.accessioned2015-11-26T11:45:28Z-
dc.description.provenanceMade available in DSpace on 2014-07-16T20:38:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-02-01en
dc.description.provenanceMade available in DSpace on 2015-11-26T11:45:28Z (GMT). No. of bitstreams: 2 S0074-02762011000100014.pdf: 790649 bytes, checksum: 8b719e23d51d6413d19e7d50b9e64f10 (MD5) S0074-02762011000100014.pdf.txt: 34512 bytes, checksum: 4430505e21d2a2254d813a8caafaa271 (MD5) Previous issue date: 2011-02-01en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/21644
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/21644-
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