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Type: Artigo de periódico
Title: Anti-adhesion And Antibacterial Activity Of Silver Nanoparticles Supported On Graphene Oxide Sheets.
Author: de Faria, Andreia Fonseca
Martinez, Diego Stéfani Teodoro
Meira, Stela Maris Meister
de Moraes, Ana Carolina Mazarin
Brandelli, Adriano
Filho, Antonio Gomes Souza
Alves, Oswaldo Luiz
Abstract: This work reports on the preparation, characterization and antibacterial activity of a nanocomposite formed from graphene oxide (GO) sheets decorated with silver nanoparticles (GO-Ag). The GO-Ag nanocomposite was prepared in the presence of AgNO3 and sodium citrate. The physicochemical characterization was performed by UV-vis spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), Raman spectroscopy and transmission electron microscopy (TEM). The average size of the silver nanoparticles anchored on the GO surface was 7.5 nm. Oxidation debris fragments (a byproduct adsorbed on the GO surface) were found to be crucial for the nucleation and growth of the silver nanoparticles. The antibacterial activity of the GO and GO-Ag nanocomposite against the microorganism Pseudomonas aeruginosa was investigated using the standard counting plate methodology. The GO dispersion showed no antibacterial activity against P. aeruginosa over the concentration range investigated. On the other hand, the GO-Ag nanocomposite displayed high biocidal activity with a minimum inhibitory concentration ranging from 2.5 to 5.0 μg/mL. The anti-biofilm activity toward P. aeruginosa adhered on stainless steel surfaces was also investigated. The results showed a 100% inhibition rate of the adhered cells after exposure to the GO-Ag nanocomposite for one hour. To the best of our knowledge, this work provides the first direct evidence that GO-Ag nanocomposites can inhibit the growth of microbial adhered cells, thus preventing the process of biofilm formation. These promising results support the idea that GO-Ag nanocomposites may be applied as antibacterial coatings material to prevent the development of biofilms in food packaging and medical devices.
Subject: Anti-bacterial Agents
Metal Nanoparticles
Microbial Sensitivity Tests
Pseudomonas Aeruginosa
Antibacterial Activity
Graphene Oxide
Pseudomonas Aeruginosa
Silver Nanoparticles
Citation: Colloids And Surfaces. B, Biointerfaces. v. 113, p. 115-24, 2014-Jan.
Rights: fechado
Identifier DOI: 10.1016/j.colsurfb.2013.08.006
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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