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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleNew Microsatellite Markers Developed From Urochloa Humidicola (poaceae) And Cross Amplification In Different Urochloa Species.pt_BR
dc.contributor.authorVigna, Bianca Bzpt_BR
dc.contributor.authorAlleoni, Guilherme Cpt_BR
dc.contributor.authorJungmann, Leticiapt_BR
dc.contributor.authordo Valle, Cacilda Bpt_BR
dc.contributor.authorde Souza, Anete Ppt_BR
unicamp.authorBianca Bz Vigna, Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas, CP 6010, Campinas, SP CEP 13083-970, Brazil. anete@unicamp.br.pt_BR
unicamp.author.externalGuilherme C Alleoni,pt
unicamp.author.externalLeticia Jungmann,pt
unicamp.author.externalCacilda B do Valle,pt
unicamp.author.externalAnete P de Souza,pt
dc.description.abstractUrochloa humidicola is a forage grass that grows in tropical regions and is recognized for its tolerance to seasonal flooding. It is a polyploid and apomictic species with high phenotypic plasticity. As molecular tools are important in facilitating the development of new cultivars and in the classification of related species, the objectives of this study were to develop new polymorphic microsatellite markers from an enriched library constructed from U. humidicola and to evaluate their transferability to other Urochloa species. Microsatellite sequences were identified from a previously constructed enriched library, and specific primers were designed for 40 loci. Isolated di-nucleotide repeat motifs were the most abundant followed by tetra-nucleotide repeats. Of the tested loci, 38 displayed polymorphism when screened across 34 polyploid Urochloa sp. genotypes, including 20 accessions and six hybrids of U. humidicola and two accessions each from U. brizantha, U. dictyoneura, U. decumbens and U. ruziziensis. The number of bands per Simple Sequence Repeat (SSR) locus ranged from one to 29 with a mean of 11.5 bands per locus. The mean Polymorphism Information Content (PIC) of all loci was 0.7136, and the mean Discrimination Power (DP) was 0.7873. Six loci amplified in all species tested. STRUCTURE analysis revealed six different allelic pools, and the genetic similarity values analyzed using Jaccard's coefficient ranged from 0.000 to 0.913. This work reports new polymorphic microsatellite markers that will be useful for breeding programs for Urochloa humidicola and other Urochloa species as well as for genetic map development, germplasm characterization, evolutionary and taxonomic studies and marker-assisted trait selection.en
dc.relation.ispartofBmc Research Notespt_BR
dc.relation.ispartofabbreviationBMC Res Notespt_BR
dc.date.issued2011pt_BR
dc.identifier.citationBmc Research Notes. v. 4, p. 523, 2011.pt_BR
dc.language.isoengpt_BR
dc.description.volume4pt_BR
dc.description.firstpage523pt_BR
dc.rightsfechadopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1756-0500pt_BR
dc.identifier.doi10.1186/1756-0500-4-523pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/22142493pt_BR
dc.date.available2015-11-27T13:22:04Z-
dc.date.accessioned2015-11-27T13:22:04Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:22:04Z (GMT). No. of bitstreams: 1 pmed_22142493.pdf: 1559154 bytes, checksum: ea50474c3079f94da6ffe2edb33f28de (MD5) Previous issue date: 2011en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199705-
dc.identifier.idPubmed22142493pt_BR
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