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Type: Artigo de periódico
Title: Tlr2 And Tlr4 Gene Promoter Methylation Status During Chronic Periodontitis.
Author: De Oliveira, Naila Francis Paulo
Andia, Denise Carleto
Planello, Aline Cristiane
Pasetto, Silvana
Marques, Marcelo Rocha
Nociti, Francisco Humberto
Line, Sérgio Roberto Peres
De Souza, Ana Paula
Abstract: The objective of this study was to analyse the status of DNA methylation in the promoter region of the toll-like receptor (TLR)2 and TLR4 genes in gingival tissue samples from healthy subjects, smokers and non-smokers affected by chronic periodontitis. Genomic DNA and total RNA were purified from gingival tissue using the TRIZOL reagent protocol. Genomic DNA was then digested by methylation-sensitive restriction enzymes, amplified by polymerase chain reaction (PCR), electrophoresed on a 10% polyacrylamide gel and stained using SYBR Gold. Real-time PCR was also performed to verify the transcript levels. The CpG dinucleotides analysed were observed to be unmethylated in the majority of DNA samples of the three groups and statistical differences were not found among groups (p>0.05). However, a trend towards methylation was observed in the TLR2 HhaI site in the samples of the periodontitis non-smoker groups. In fact, the analysis of all CpG sites together shows which complete methylation is observed in the shortest level in the samples of periodontitis non-smoker group. The analysis of transcript levels demonstrated no difference among groups (p>0.05). The results demonstrated major unmethylation of the TLR4 gene promoter in all groups. However, the results for the TLR2 gene promoter are inconclusive; this gene was found as a mosaic of methylated and unmethylated DNA in the majority of samples of the three groups and we also observed a trend towards the DNA methylation of CpG sites recognized by the HhaI enzyme.
Subject: Adult
Case-control Studies
Chi-square Distribution
Chronic Periodontitis
Cpg Islands
Dna Methylation
Middle Aged
Promoter Regions, Genetic
Reverse Transcriptase Polymerase Chain Reaction
Statistics, Nonparametric
Toll-like Receptor 2
Toll-like Receptor 4
Citation: Journal Of Clinical Periodontology. v. 38, n. 11, p. 975-83, 2011-Nov.
Rights: fechado
Identifier DOI: 10.1111/j.1600-051X.2011.01765.x
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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