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Type: Artigo de periódico
Title: Purification And Structural Characterization Of Fengycin Homologues Produced By Bacillus Subtilis Lsfm-05 Grown On Raw Glycerol.
Author: de Faria, Andreia Fonseca
Stéfani, Diego
Vaz, Boniek Gontijo
Silva, Ísis Serrano
Garcia, Jerusa Simone
Eberlin, Marcos N
Grossman, Matthew James
Alves, Oswaldo Luiz
Durrant, Lucia Regina
Abstract: Raw glycerol is a byproduct of biodiesel production that currently has low to negative value for biodiesel producers. One option for increasing the value of raw glycerol is to use it as a feedstock for microbial production. Bacillus subtilis LSFM 05 was used for the production of fengycin in a mineral medium containing raw glycerol as the sole carbon source. Fengycin was isolated by acid precipitation at pH 2 and purified by silica gel column chromatography and characterized using electrospray ionization (ESI) Fourier transform ion cyclotron resonance mass spectrometry (ESI FT-ICR MS) with collision-induced dissociation (CID). The mass spectrum revealed the presence of the ions of m/z 1,435.7, 1,449.9, 1,463.8, 1,477.8, 1,491.8 and 1,505.8, which were further fragmented by ESI-MS/MS. The CID profile showed the presence of a series of ions (m/z 1,080 and 966) and (m/z 1,108 and 994) that represented the different fengycin homologues A and B, respectively. Fengycin homologues A and B are variants that differ at position 6 of the peptide moiety, having either Ala or Val residues, respectively. Mass spectrometry analyses identified four fengycin A and three fengycin B variants with fatty acid components containing 14-17 carbons. These results demonstrate that raw glycerol can be used as feedstock to produce fengycin, and additional work should focus on the optimization of process conditions to increase productivity.
Subject: Bacillus Subtilis
Spectrometry, Mass, Electrospray Ionization
Spectroscopy, Fourier Transform Infrared
Surface-active Agents
Tandem Mass Spectrometry
Citation: Journal Of Industrial Microbiology & Biotechnology. v. 38, n. 7, p. 863-71, 2011-Jul.
Rights: fechado
Identifier DOI: 10.1007/s10295-011-0980-1
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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