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Type: Artigo de periódico
Title: Cytomegalovirus Frequency In Neonatal Intrahepatic Cholestasis Determined By Serology, Histology, Immunohistochemistry And Pcr.
Author: Bellomo-Brandao, Maria Angela
Andrade, Paula D
Costa, Sandra C B
Escanhoela, Cecilia A F
Vassallo, Jose
Porta, Gilda
De Tommaso, Adriana M A
Hessel, Gabriel
Abstract: To determine cytomegalovirus (CMV) frequency in neonatal intrahepatic cholestasis by serology, histological revision (searching for cytomegalic cells), immunohistochemistry, and polymerase chain reaction (PCR), and to verify the relationships among these methods. The study comprised 101 non-consecutive infants submitted for hepatic biopsy between March 1982 and December 2005. Serological results were obtained from the patient's files and the other methods were performed on paraffin-embedded liver samples from hepatic biopsies. The following statistical measures were calculated: frequency, sensibility, specific positive predictive value, negative predictive value, and accuracy. The frequencies of positive results were as follows: serology, 7/64 (11%); histological revision, 0/84; immunohistochemistry, 1/44 (2%), and PCR, 6/77 (8%). Only one patient had positive immunohistochemical findings and a positive PCR. The following statistical measures were calculated between PCR and serology: sensitivity, 33.3%; specificity, 88.89%; positive predictive value, 28.57%; negative predictive value, 90.91%; and accuracy, 82.35%. The frequency of positive CMV varied among the tests. Serology presented the highest positive frequency. When compared to PCR, the sensitivity and positive predictive value of serology were low.
Subject: Cholestasis, Intrahepatic
Cytomegalovirus Infections
Infant, Newborn
Polymerase Chain Reaction
Retrospective Studies
Serologic Tests
Viral Load
Citation: World Journal Of Gastroenterology : Wjg. v. 15, n. 27, p. 3411-6, 2009-Jul.
Rights: aberto
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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