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Type: Artigo de periódico
Title: Different Urea Stoichiometries Between The Dissociation And Denaturation Of Tobacco Mosaic Virus As Probed By Hydrostatic Pressure.
Author: Santos, Jose L R
Aparicio, Ricardo
Joekes, Inés
Silva, Jerson L
Bispo, Jose A C
Bonafe, Carlos F S
Abstract: Viruses are very efficient self-assembly structures, but little is understood about the thermodynamics governing their directed assembly. At higher levels of pressure or when pressure is combined with urea, denaturation occurs. For a better understanding of such processes, we investigated the apparent thermodynamic parameters of dissociation and denaturation by assuming a steady-state condition. These processes can be measured considering the decrease of light scattering of a viral solution due to the dissociation process, and the red shift of the fluorescence emission spectra, that occurs with the denaturation process. We determined the apparent urea stoichiometry considering the equilibrium reaction of TMV dissociation and subunit denaturation, which furnished, respectively, 1.53 and 11.1 mol of urea/mol of TMV subunit. The denaturation and dissociation conditions were arrived in a near reversible pathway, allowing the determination of thermodynamic parameters. Gel filtration HPLC, electron microscopy and circular dichroism confirmed the dissociation and denaturation processes. Based on spectroscopic results from earlier papers, the calculation of the apparent urea stoichiometry of dissociation and denaturation of several other viruses resulted in similar values, suggesting a similar virus-urea interaction among these systems.
Subject: Chromatography, Gel
Chromatography, High Pressure Liquid
Hydrostatic Pressure
Microscopy, Electron, Transmission
Molecular Conformation
Tobacco Mosaic Virus
Citation: Biophysical Chemistry. v. 134, n. 3, p. 214-24, 2008-May.
Rights: fechado
Identifier DOI: 10.1016/j.bpc.2008.02.010
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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