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Type: Artigo de periódico
Title: Inflammatory Responses Induced In Mice By Lectin From Talisia Esculenta Seeds.
Author: Freire, M G M
Desouza, I A
Silva, A C M
Macedo, M L R
Lima, M S
Tamashiro, W M S C
Antunes, E
Marangoni, S
Abstract: A novel lectin from Talisia esculenta seeds (TEL) has recently been purified and characterized. In this study we investigated the proinflammatory activity of TEL in mice using both the air-pouch and peritoneal cavity as well as paw oedema models. TEL (10-40 microg) induced significant neutrophil and mononuclear cell recruitment when injected into either mouse air-pouch or peritoneal cavity. The neutrophil accumulation into the air-pouch was dose- and time-dependent with a maximal response at 16 h, returning to control levels at 72 h whereas maximal mononuclear cell accumulation was observed at 24 h after TEL injection. The same profile of neutrophil accumulation was observed when this lectin was injected into mouse peritoneal cavity, although the maximal mononuclear cell recruitment was observed 48 h after TEL injection. Additionally, TEL (12.5-200 microg/paw) caused a dose-dependent mice paw, as evaluated at 4 h after the lectin injection. D-mannose, better than D-glucose, significantly inhibited TEL-induced neutrophil migration into the peritoneal cavity or air-pouch. D-galactose had no effect on TEL-induced neutrophil migration in either cavity studied. On the other hand, D-mannose slightly inhibited the TEL-induced paw oedema, whereas neither D-glucose nor D-galactose affected this phenomenon. In conclusion, our data show that TEL induces neutrophil and mononuclear cell accumulation by a mechanism related to their specific sugar-binding properties.
Subject: Analysis Of Variance
Chemotaxis, Leukocyte
Dose-response Relationship, Immunologic
Leukocytes, Mononuclear
Peritoneal Cavity
Plant Lectins
Citation: Toxicon : Official Journal Of The International Society On Toxinology. v. 42, n. 3, p. 275-80, 2003-Sep.
Rights: fechado
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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