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Type: Artigo de periódico
Title: Inhibition Of Human Gingival Gelatinases (mmp-2 And Mmp-9) By Metal Salts.
Author: de Souza, A P
Gerlach, R F
Line, S R
Abstract: The interaction between metal ions and the oral environment is a major subject matter in dental research. Matrix metalloproteinases (MMPs) have been implicated in several pathologic oral processes such as periodontal tissue destruction, root caries, tumour invasion and temporomandibular joint disorders. The aim of this work was to test the effect of Zn, Cu, Sn and Hg ions on the activity of the major gingival gelatinolytic MMPs. Gingival explants were cultured overnight in DMEM and the activity of secreted enzymes was analyzed by gelatin zymography in buffers containing different metal ion concentrations. The major gelatinolytic proteinases present in the conditioned media were characterized as MMP-2 and MMP-9 by immunoprecipitation with specific antibodies. The eletrophoretic bands were scanned and the transmittance values were analyzed with the Sigmagel software (Sigma). ZnSO4 was a strong inhibitor of MMP-2 (I50 = 15 microM) and MMP-9 (I50 = 40 microM), whereas CuSO4, HgSO4 and SnCl2 showed less efficient inhibition potential. Our findings show that the activity of oral tissue MMPs may be modulated by metal ions present in the oral environment. Therefore, the accumulation of metals in connective tissue may interfere with the formation and resorption of the extracellular matrix components.
Subject: Adult
Cells, Cultured
Copper Sulfate
Culture Media, Conditioned
Electrophoresis, Polyacrylamide Gel
Enzyme Inhibitors
Extracellular Matrix
Image Processing, Computer-assisted
Matrix Metalloproteinase Inhibitors
Mercury Compounds
Middle Aged
Precipitin Tests
Tin Compounds
Zinc Sulfate
Citation: Dental Materials : Official Publication Of The Academy Of Dental Materials. v. 16, n. 2, p. 103-8, 2000-Mar.
Rights: fechado
Date Issue: 2000
Appears in Collections:Unicamp - Artigos e Outros Documentos

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