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dc.typeArtigo de periódicopt_BR
dc.titleMeasuring Electrical And Mechanical Properties Of Red Blood Cells With Double Optical Tweezerspt_BR
dc.contributor.authorFontes A.pt_BR
dc.contributor.authorFernandes H.P.pt_BR
dc.contributor.authorDe Thomaz A.A.pt_BR
dc.contributor.authorBarbosa L.C.pt_BR
dc.contributor.authorBarjas-Castro M.L.pt_BR
dc.contributor.authorCesar C.L.pt_BR
unicamp.authorFernandes, H.P., Universidade Estadual de Campinas (UNICAMP), Department of Pharmacology, Faculty of Medical Sciences, 480 Carlos Chagas Street, 13083-970, Campinas, SP, Brazilpt_BR
unicamp.authorDe Thomaz, A.A., Universidade Estadual de Campinas (UNICAMP), Departamento de Eletrônica Quântica, Instituto de Física Gleb Wataghin, 13083-970, Campinas-SP, Brazilpt_BR
unicamp.authorBarbosa, L.C., Universidade Estadual de Campinas (UNICAMP), Departamento de Eletrônica Quântica, Instituto de Física Gleb Wataghin, 13083-970, Campinas-SP, Brazilpt_BR
unicamp.authorBarjas-Castro, M.L., Universidade Estadual de Campinas (UNICAMP), Hematology and Transfusion Center, 480 Carlos Chagas Street, 13083-970, Campinas, SP, Brazilpt_BR
unicamp.authorCesar, C.L., Universidade Estadual de Campinas (UNICAMP), Departamento de Eletrônica Quântica, Instituto de Física Gleb Wataghin, 13083-970, Campinas-SP, Brazilpt_BR, A., Universidade Federal de Pernambuco (UFPE), Cidade Universitária, Departamento de Biofísica e Radiobiologia, Av. Professor Moraes Rego, 50670-901, Recife-PE, Brazilpt
dc.description.abstractRed blood cell (RBC) aggregation in the blood stream is prevented by the zeta potential created by its negatively charged membrane. There are techniques, however, to decrease the zeta potential and allow cell agglutination, which are the basis of most of antigen-antibody tests used in immunohematology. We propose the use of optical tweezers to measure membrane viscosity, adhesion, zeta potential, and the double layer thickness of charges (DLT) formed around the cell in an electrolytic solution. For the membrane viscosity experiment, we trap a bead attached to RBCs and measure the force to slide one RBC over the other as a function of the velocity. Adhesion is quantified by displacing two RBCs apart until disagglutination. The DLT is measured using the force on the bead attached to a single RBC in response to an applied voltage. The zeta potential is obtained by measuring the terminal velocity after releasing the RBC from the trap at the last applied voltage. We believe that the methodology proposed here can provide information about agglutination, help to improve the tests usually performed in transfusion services, and be applied for zeta potential measurements in other samples. © 2008 Society of Photo-Optical Instrumentation Engineers.en
dc.relation.ispartofJournal of Biomedical Opticspt_BR
dc.identifier.citationJournal Of Biomedical Optics. , v. 13, n. 1, p. - , 2008.pt_BR
dc.description.provenanceMade available in DSpace on 2015-06-30T19:35:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2008en
dc.description.provenanceMade available in DSpace on 2015-11-26T14:46:01Z (GMT). No. of bitstreams: 0 Previous issue date: 2008en
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