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Type: Artigo de periódico
Title: Expression Of Hypoxia-inducible Factor 1α In Mononuclear Phagocytes Infected With Leishmania Amazonensis
Author: Degrossoli A.
Bosetto M.C.
Lima C.B.C.
Giorgio S.
Abstract: Increasing evidence indicates that hypoxia-inducible factor 1α (HIF-1α) can be upregulated in different cell types by nonhypoxic stimuli such as growth factors, cytokines, nitric oxide, lipopolysaccharides and a range of infectious microorganisms. In this study, the ability of the following mononuclear phagocytes to express HIF-1α is reported: mouse macrophages (mMΦ), human macrophages (hMΦ) and human dendritic cells (DC), parasitized in vitro with Leishmania amazonensis; as assessed by immunofluorescence microscopy. A logical explanation for HIF-1α expression might be that the mononuclear phagocytes became hypoxic after L. amazonensis infection. Using the hypoxia marker pimonidazole, observation revealed that L. amazonensis-infected cells were not hypoxic. In addition, experiments using a HIF-1α inhibitor, CdCl2, to treat L. amazonensis-infected macrophage cultures showed reduced parasite survival. These studies indicated that HIF-1α could play a role in adaptative and immune responses of mononuclear phagocytes presenting infection by the parasite L. amazonensis. © 2007 Elsevier B.V. All rights reserved.
Citation: Immunology Letters. , v. 114, n. 2, p. 119 - 125, 2007.
Rights: fechado
Identifier DOI: 10.1016/j.imlet.2007.09.009
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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