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Type: Artigo de evento
Title: Molecular Differentiation Of Leishmania Protozoarium Using Cds Quantum Dots As Biolabels
Author: Santos B.S.
De Farias P.M.A.
De Menezes F.D.
Mariano E.L.
Ferreira R.D.C.
Giorgio S.
Bosetto M.C.
Ayres D.C.
Lima P.M.
Fontes A.
De Thomas A.A.
Cesar C.L.
Abstract: In this work we applied core-shell CdS/Cd(OH)2 quantum dots (QDs) as fluorescent labels in the Leishmania amazonensis protozoarium. The nanocrystals (8-9 nm) are obtained via colloidal synthesis in aqueous medium, with final pH=7 using sodium polyphosphate as the stabilizing agent. The surface of the particles is passivated with a cadmium hydroxide shell and the particle surface is functionalized with glutaraldehyde. The functionalized and non-functionalized particles were conjugated to Leishmania organisms in the promastigote form. The marked live organisms were visualized using confocal microscopy. The systems exhibit a differentiation of the emission color for the functionalized and non-functionalized particles suggesting different chemical interactions with the promastigote moieties. Two photon emision spectra (λexc=795nm) were obtained for the promastigotes labeled with the functionalized QDs showing a significant spectral change compared to the original QDs suspension. These spectral changes are discussed in terms of the possible energy deactivation processes.
Citation: Progress In Biomedical Optics And Imaging - Proceedings Of Spie. , v. 6097, n. , p. - , 2006.
Rights: fechado
Identifier DOI: 10.1117/12.646912
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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